Diagnostic Support

The Department provides laboratory diagnosis of parasitic diseases to the following hospitals and centres and any other health institutions needing diagnostic assistance.

Disease Category

Test

Specimen
(Required Volume/Quantity)

Special Facts

Blood Borne Parasites

Malaria

Thick smear for the detection of parasites

Intravenous blood (0.5ml) or finger prick blood (100ul)

IV blood in an EDTA bottle, finger prick blood can be sent in a capillary tube

Malaria

Thin smear for species identification

Intravenous blood (0.5ml) or finger prick blood (100ul)

IV blood in an EDTA bottle, finger prick blood can be sent in a capillary tube

Filariasis

Thick smear for the detection of parasites

Intravenous blood (0.5ml) or finger prick blood (100ul)

IV blood in an EDTA bottle, finger prick blood can be sent in a capillary tube

Filariasis

Thin smear for species identification

Intravenous blood (0.5ml) or finger prick blood (100ul)

IV blood in an EDTA bottle, finger prick blood can be sent in a capillary tube

Leishmaniasis (cutaeneous)

Microscopy of stained Smear

Slit Skin Smear

-

Leishmaniasis (cutaeneous)

PCR

Skin Biopsy

-

Leishmaniasis (Visceral)

Rapid Immunochromatographic test (ICT)

Serum (0.5ml)

In a plain bottle

Leishmaniasis (Visceral)

Anti-Leishmania antibody

Serum (0.5ml)

In a plain bottle

Leishmaniasis (Visceral)

Microscopy of stained Smear for

Intravenous blood (2ml). The test is done using the buffy coat.

In an EDTA bottle, transported preferably in ice

Leishmaniasis (Visceral)

PCR

bone marrow aspirate (0.5 ml or less)

In an EDTA bottle, must be transported in ice

Parasites of the Intestinal Tract

Nematode worm infections

Identification of parasitic worms

Macroscopic specimens of worms

In formal saline

Taeniasis and other cestode worm infections

Identification of parasitic worms

Macroscopic specimens of worms

In formal saline

Amobiasis, balantidiasis, giaridiasis, non pathogenic protozans, nematode worm infections and cestode worm infections

Stool Full Report (AOC)

Faeces (1 g)

In a clean wide mouth container

Nematode worm infections

Kato Katz (cellophane thick smear) for nematode egg quantification

Faeces (1 g)

In a clean wide mouth container

Cryptosporidiosis

Modified Zeil Neelson stained smear

Faeces (1 g)

In a clean wide mouth container

Enterobius (pin worm) infections

Graham’s scotch tape method

Graham’s scotch taped specimen obtained from peri anal region

Tape pasted on a clean glass

Strongyloidiasis

Stool culture (Harada Mori)

Faeces (1 g)

In a clean wide mouth container

Strongyloidiasis

Stool culture (agar plate)

Faeces (1 g)

In a clean wide mouth container

Parasites Found in Other Sites

Trichomoniasis

Wet smear

Vaginal swab/early morning urine sample

Vaginal swab dipped in normal saline, Urine in a clean container

Trichomoniasis

Culture

Vaginal swab/early morning urine sample

Vaginal swab dipped in normal saline, urine in a clean container

Hydatic disease (Echinococcosis)

Microscopy of the cyst fluid

Cyst fluid (1ml)

In a clean bottle

Dirofilaria (zoonotic filariasis)

Microscopy of stained Smear

Biopsy sample or worm if extracted

In formal saline

Disease causing vectors

Examination under the dissecting microscope and dissection of the specimen as required

Entomological specimens

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